The invention provides a method for extraction separation of chlorogenic acid from Chinese wolfberry residue meal. Th method comprises the following operation steps of: (1) taking oil extracted Chinese wolfberry residue meal, taking ethanol as an entrainer to perform supercritical carbon dioxide extraction, and reserving the extract for standby use; and (2) loading the supercritical carbon dioxide extraction extract on macroporous adsorption resin, preferably D101 type macroporous resin, conducting elution with water and 15% (V/V) ethanol in order, collecting the 15% (V/V) ethanol eluted parts, and carrying out concentration and drying, thus obtaining chlorogenic acid. The raw material adopted by the invention is oil extracted Chinese wolfberry residue meal, the industrial waste is reutilized, the cost is low, and the added value of Chinese wolfberry is greatly increased. Thus, the method provided by the invention significantly enhances the comprehensive utilization rate of Chinese wolfberry resources.