The invention provides egg oil and a preparation method and an application thereof. Specifically, the invention provides a preparation method of the egg oil. The prepared egg oil is a transparent liquid, can have mutual solubility with water, mainly contains cholesterol (TG) lipoprotein (including high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C)) and mutually-combined triglyceride (TG) and lipoprotein and has high hydrophilicity. In addition, the preparation method is simple and suitable for industrial production.