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发明公开
CN104342406A 热稳定性增强的甲酸脱氢酶突变体及其制备方法 有权 转让

热稳定性增强的甲酸脱氢酶突变体及其制备方法
摘要：
本发明涉及一种热稳定性增强的甲酸脱氢酶突变体及其制备方法，该突变体源于博伊丁假丝酵母(Candida boidinii)的野生型甲酸脱氢酶，能够催化辅酶NADH循环再生，与野生型甲酸脱氢酶相比表现出更高的热稳定性，具有I98V、V152I、A154D、D158R中的一个或多个突变特征。本发明的重组甲酸脱氢酶突变体具有更好的热稳定性，适用于较高温度下的NADH的循环再生，与目前发现的甲酸脱氢酶相比，本发明的甲酸脱氢酶突变体在50℃－55℃之间活性没有明显降低，具有更好的热稳定性，因此更适合于工业化应用。
摘要（英）：

The invention relates to a thermostability enhanced formate dehydrogenase mutant and a preparation method thereof. The mutant is derived from wild type formate dehydrogenase of Candida boidinii, can catalyze coenzyme NADH cyclic regeneration, shows higher thermostability compared with the wild type formate dehydrogenase, and has one or more mutation characteristics of I98V, V152I, A154D and D158R. The recombinant formate dehydrogenase mutant provided by the invention has better thermostability, and is suitable for cyclic regeneration of NADH under high temperature. Compared with the formate dehydrogenase discovered so far, the formate dehydrogenase mutant has the activity that is not significantly reduced in the range of 50DEG C-55DEG C, and has better thermostability, thus being more suitable for industrial application.

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