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发明公开
CN104356062A 对羟基苯甲醛缩异烟肼的制备方法及其应用 无效 - 驳回

对羟基苯甲醛缩异烟肼的制备方法及其应用
摘要：
本发明公开了一种对羟基苯甲醛缩异烟肼的制备方法及其应用，属于植物病害防治领域。本发明中利用对羟基苯甲醛缩异烟肼，采用菌丝生长速率法，研究其对黄瓜猝倒病菌和水稻稻瘟病菌的抑制效果。将药剂浓度设置为1、2、4、6、8μg/ml五个梯度进行试验，以生长速率法测定菌落抑制效果，通过对菌落直径数据的处理得到病菌的致死中浓度EC50和抑制率。以EC50值的角度进行比较，病菌对对羟基苯甲醛缩异烟肼的敏感程度为黄瓜猝倒病菌>水稻稻瘟病菌。实验结果证明，对羟基苯甲醛缩异烟肼对两种病菌均有抑制效果，其中对黄瓜猝倒病菌的抑制效果最好。
摘要（英）：

The invention discloses a preparation method of p-hydroxybenzaldehyde condensed isoniazide and application thereof, and belongs to the field of plant disease control. According to the method, p-hydroxybenzaldehyde condensed isoniazide is utilized to research the inhibitory effect on cucumber damping-off fungi and phyricularia grisea by p-hydroxybenzaldehyde condensed isoniazide through a mycelial growth rate method. Agent concentration is set to be five gradients which are 1 microgram/ml, 2 microgram/ml, 4 microgram/ml, 6 microgram/ml and 8 microgram/ml to perform tests, the bacterial colony inhibitory effect is measured through the growth rate method, and the median lethal concentration EC50 and inhibition ratio of germs are obtained by processing diameter data of the bacterial colony. Being contrasted from the point of the EC50 value, the sensitive degree of germs on p-hydroxybenzaldehyde condensed isoniazide is cucumber damping-off fungi and phyricularia grisea. Experimental results show that p-hydroxybenzaldehyde condensed isoniazide has an inhibitory effect on both cucumber damping-off fungi and phyricularia grisea, wherein the inhibitory effect on cucumber damping-off fungi is the best.

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