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发明公开
CN104357567A miR-193实时荧光定量PCR检测方法 无效 - 驳回

miR-193实时荧光定量PCR检测方法
摘要：
本发明公开了一种miR-193实时荧光定量PCR检测方法，包括血清中总RNA的提取步骤、逆转录合成cDNA反应、荧光定量PCR扩增反应、荧光定量PCR产物经2%琼脂糖凝胶电泳等步骤。本发明易操作、准确，所采用的实时荧光定量PCR检测方法，巧妙的运用PCR技术的DNA高效扩增，高效、灵敏的检测miR-193。
摘要（英）：

The invention discloses a miR-193 detecting method based on real-time fluorescence quantitative PCR (polymerase chain reaction). The method includes the following steps: extracting total RNA from serum, synthesizing cDNA via reverse transcription, carrying out fluorogenic quantitative PCR amplification, performing agarose gel electrophoresis on fluorogenic quantitative PCR products, wherein the concentration of the agarose gel is 2%, and so on. The miR-193 detecting method based on real-time fluorescence quantitative PCR is easy to operate and accurate, and can be used for efficiently and sensitively detecting miR-193 as the adopted real-time fluorescence quantitative PCR detection method ingeniously utilizes DNA efficient amplification based on the PCR technology.

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