The invention provides an all-direction wire harness fatigue test fixture. The all-direction wire harness fatigue test fixture is formed by rotatably connecting a first-direction rotating module, a second-direction rotating module and a third-direction rotating module in sequence, wherein the first-direction rotating module comprises a base and a shaft block, and the base and the shaft block are rotatably and fixedly connected through a screw shaft arranged at the bottom of the base and the bottom of the shaft block; the second-direction rotating module comprises a shaft block and a rotating shaft column, and the rotating shaft column penetrates through the shaft block and is rotatably fixed on the shaft block; the third-direction rotating module comprises a rotating shaft column and a rotating piece, and the rotating shaft column is provided with a stud allowing the rotating piece to pass and be rotatably fixed. According to the all-direction wire harness fatigue test fixture, the rotating angle can be adjusted in three directions so that all-direction rotation can be achieved, and various installation conditions of a wire harness in a car can be simulated; accordingly, the data, obtained from a vibration fatigue test, of the wire harness are more accurate and reliable.