The invention discloses an indirect competitive enzyme-linked adapter analysis method (ELAA) for detection of residual terramycin in food, and belongs to the field of food safety analysis. Through use of artificially synthesized terramycin adapter for replacement of antibody, the indirect competitive enzyme-linked adapter analysis method (ELAA) of terramycin is established. The linear range of the standard curve is 0.5-50ng / mL, the half inhibitory amount (IC50) is 6.30ng / mL, and the sensitivity (IC90) is 0.5ng / mL. After addition of terramycin with different concentrations into poultry meat, aquatic products, milk, milk powder and honey matrixes, ELAA method is used for detection, the adding standard recovery rate is 62.1%-102%, and the coefficient of variation (CV) is less than 20%. The method of the invention not only has simple operation, fast detection large analysis capacity and other advantages of ELISA (enzyme-linked immuno sorbent assay), but also overcomes the cumbersome antibody preparation process, long cycle and high cost problems, is suitable for mass sample test and on-site monitoring, and has important practical significance.