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发明公开
CN104374912A 一种百合隐症病毒、斑驳病毒和黄瓜花叶病毒双向胶体金免疫层析速测卡及制备方法 有权 转让

一种百合隐症病毒、斑驳病毒和黄瓜花叶病毒双向胶体金免疫层析速测卡及制备方法
摘要：
一种百合隐症病毒、百合斑驳病毒和百合黄瓜花叶病毒双向复合胶体金免疫层析速测卡及制备方法，采用双向免疫层析技术，该速测卡可一次性同时快速检测LSV、LMoV和CMV，无需任何仪器和设备，较LSV和LMoV单一或LSV+LMoV复合胶体金免疫层析试剂卡，本发明速测卡检测效率更高，检测成本进一步降低，一次性检测病毒种类更齐全；此外，其操作简便，携带方便，检测快速、特异性强、重复性好，相对于PCR方法，假阳性率低于1％。可见本发明特别适用于在田间推广应用，并且可靠性很高。
摘要（英）：

The invention discloses a lily hidden disease virus, mottle virus and cucumber mosaic virus bidirectional colloidal gold immunity chromatography detection card and a preparation method thereof. The lily hidden disease virus, mottle virus and cucumber mosaic virus bidirectional colloidal gold immunity chromatography detection card utilizes a bidirectional immunity chromatography technology and can simultaneously and fast detect LSV, LMoV and CMV by one step without any apparatus and equipment. Compared with the existing LSV, or LMoV or LSV-LMoV compound colloidal gold immunity chromatography detection card, the lily hidden disease virus, mottle virus and cucumber mosaic virus bidirectional colloidal gold immunity chromatography detection card has higher detection efficiency, further reduces a detection cost and can detect various types of viruses by one step. The lily hidden disease virus, mottle virus and cucumber mosaic virus bidirectional colloidal gold immunity chromatography detection card is convenient for operation and carrying, has a fast detection rate, good singularity and good repeatability and has a false positive rate 1% less than that of the PCR method. The lily hidden disease virus, mottle virus and cucumber mosaic virus bidirectional colloidal gold immunity chromatography detection card is especially suitable for popularization in field and has high reliability.

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