The invention discloses a production method of organic chlorella enriched in trace elements. The production method comprises the following steps of preparing a biological anaerobic fermentation culture medium, carrying out anaerobic fermentation on a liquid organic fertilizer, producing the organic chlorella and the like. The production method is suitable for experimental production and large-scale production of the chlorella of a level higher than pilot test level. By virtue of the anaerobic fermentation method, the production of the liquid organic fertilizer of the chlorella is achieved, the enrichment and organization of the trace elements are achieved during the preparation of the chlorella cultivation culture medium, the chlorella is cultivated by virtue of the liquid fertilizer enriched in organized trace elements and thus organic chlorella products enriched in various trace elements can be obtained, since various trace elements contained in the chlorella products are organized, the trace elements are easily absorbed by the human body. The chlorella products are prepared from biomass wastes as source materials and thus the recycling and high-valued utilization of the wastes are achieved and the organization of the production process of the chlorella brings environmental and economic double effects.