The invention relates to a five-step method for inducing lilium sulphureum bulbil callus to re-differentiate aseptic seedlings. According to the invention, lilium sulphureum bulbils are adopted as explants; through transition culturing, pollution-free explants are transferred to a callus culturing medium; a proliferation culturing medium is used for proliferating the callus; clustered buds are differentiated from the callus; and the clustered buds are transferred to a rooting and seedling-developing culturing medium. In each step, optimization is carried out upon different explant growth regulator types, concentrations and ratios. With the method, pollution-free explants can be obtained. Callus can be induced in 7 days, wherein an induction rate reaches 100%. The callus can be sub-cultured more than 10 times. Clustered buds can be induced in 15 days, wherein an induction rate reaches 98%. Robust aseptic seedlings with roots can be obtained in 14 days, wherein an induction rate reaches 100%. Compared to conventional propagation method of sowing, bulbil and scale cutting, and the like, the method provided by the invention has the advantages of short period, low cost, and high propagation coefficient.