The invention discloses a lentinus edodes culture material for highly producing umami. The lentinus edodes culture material comprises the following components by weight percent: 10-30% of pleurotus eryngii wastes, 49-69% of wood flour, 20% of bran and 1% of gypsum powder. Lentinus edodes cultured by the lentinus edodes culture material is high in delicious substance content and high in single-rod yield.