The invention belongs to the field of domestic silkworm variety breeding and particularly relates to a breeding method and application of a robust self-cocoon-emerging stripe double sex-limited marking domestic silkworm variety. The breeding method comprises the steps of introducing stripe sex-limited gene into a silkworm variety for production, accordingly improve the comprehensive economic character of the stripe sex-limited gene, introducing the stripe sex-limited gene into short-growth-duration blood relationship through hybridization, and adopting a silkworm moth self-cocoon-emerging method and the like to conduct hybridization and domestication improvement on the stripe sex-limited variety. The male and female silkworms of the bred variety can be separated at a larval phase by utilizing the stripe sex-limited characteristic of the silkworms, silkworm moths can break cocoons and emerge by themselves at a pupal stage, and self-cocoon-emerging rate is higher than 95%. The stripe double sex-limited marking domestic silkworm variety bred by means of the breeding method has the advantages of being short in growth duration, strong and healthy in constitution, excellent in cocoon silk quality, high in self-cocoon-emerging rate and the like.