The invention discloses a double-sided multi-station three-stage biosafety cabinet which comprises a cabinet body, a bracket, material transmitting boxes respectively arranged at the left end and the right end of the cabinet body, an air supply filtering device arranged in the middle of the top of the cabinet body, and exhaust air filtering devices respectively arranged at the front side and the rear side of the top of the cabinet body; the biosafety cabinet also comprises transparent station surfaces respectively arranged on the front side surface and the rear side surface of the cabinet body; each transparent station surface is connected with at least two operating rubber gloves in a sealing way, and the operating rubber gloves are arranged in the cabinet body. The double-sided station design structure of arranging operating stations at the front side and the rear side is adopted, and the plurality of operating stations of the rubber gloves are arranged on the station surfaces, so that the equipment resource of the biosafety cabinet is utilized to the utmost extent in the actual operation process, the production output efficiency is improved, and the cost is lowered; therefore, the double-sided multi-station three-stage biosafety cabinet is widely popularized and used in the practical application in industry.