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发明公开
CN104450729A 猪肉质性状相关WNT10B基因分子标记的克隆及应用 失效 - 权利终止

猪肉质性状相关WNT10B基因分子标记的克隆及应用
摘要：
本发明属于家畜分子分子生物学技术领域，具体涉及猪肉质性状相关WNT10B基因分子标记的克隆及应用。本发明所述的分子标记由猪WNT10B基因克隆得到，其WNT10B分子标记的序列如SEQ ID NO：1所示。WNT10B基因的多态性是利用比较基因组学方法根据人的WNT10B基因序列设计引物，以猪的基因组DNA为模板扩增，扩增片段利用测序筛选SNP，并利用PCR－RFLP进行基因分型，发现第552bp处有一个A/G的碱基突变，导致PCR-RFLP-Sal I多态性，为猪肉质性状标记辅助选择提供了新的分子标记，可利用该标记检测中外猪种的情况。
摘要（英）：

The invention belongs to the field of the livestock molecule molecular biological technique and particularly relates to a clone and the application of a pig meat quality character relevance WNT10B gene molecular mark. The molecular mark is obtained by cloning pig WNT10B genes, and the sequence of the WNT10B molecular mark of the WNT10B gene is shown in SEQ ID NO:1. According to the polymorphism of the WNT10B, a primer is designed according to the WNT10B gene sequence of people with a genomics method, the genomic DNA of pigs is used as the template for amplification, SNP is screened by amplified fragments through sequencing, PCR-RFLP is used for conducting gene typing, a basic group mutation is found at the 552 bp position, polymorphism of PCR-RFLP-Sal I is caused, a new molecular mark is provided for auxiliary selection of the pig meat quality character mark, and the mark can be used for detecting the conditions of domestic and foreign pig breeds.

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