A non-volatile memory device is provided. The non-volatile memory device includes a substrate area, two storage units, a spacer structure and two control units. The storage units include two anti-fuse gates each having a gate dielectric layer between the anti-fuse gate and the substrate area and two diffusion areas. The spacer structure is formed on the substrate area and between the two anti-fuse gates and contacts thereto. Each of the diffusion areas is a first doping area doped with a first type dopant contacting one of the two anti-fuse gates. Each of the control units includes a select gate formed on the substrate area and a second doping area. A first side of the select gate contacts one of the diffusion areas of the storage unit. The second doping area is doped with the first type dopant and contacts a second side of the select gate.