The embodiment of the invention discloses a laminated organic LED, a display device and a preparation method of the laminated organic LED. The laminated organic LED comprises at least two light-emitting units which are arrayed in a laminated mode, and charge generation layers which are arranged between every two adjacent light-emitting units. Each charge generation layer comprises a first material layer, an electron injection layer arranged on the first material layer and a second material layer arranged on the electron injection layer. According to the laminated organic LED and the preparation method of the laminated organic LED, a good electron injection effect can be achieved even under the condition that an inverted laminated organic LED (a lower electrode is a negative electrode) is manufactured.