The invention aims at providing a 1000 MW electric generator stator transport interface part assembling method which is applied to preparation work before 1000 MW electric generator stator transport. By means of the method, the problems and potential unsafe hazards of an existing installation method are eliminated, the accuracy of the assembling relationship and assembling size is ensured, the relationship of an electric generator stator and a vehicle is correct, the structure combination deformation meets the expected requirements, the influences of abnormal gaps on stress distribution changes are eliminated, the correct assembling relationship between a connecting rod and a spherical washer set is ensured, the abnormal initial stress of root parts of ear plates is eliminated, and it is ensured that the correct initial stress of the connecting rod is obtained. Due to the application of a measurement bracket, the bending sink amount and changes of a machine base of the electric generator stator are accurately monitored, the real-time data for ensuring the transport safety are obtained, and guarantees are provided for subsequent correct vehicle combination, safe transport and transport monitoring.