The invention provides a method for testing flow velocity induction of fish, in particular a method for testing the flow velocity induction of the fish during constructing an ecological fishway, and belongs to the field of test for ecological environmental protection in water conservancy projects. The method for testing the flow velocity induction of the fish comprises the steps of preparing a fish swimming testing device; arranging a testing section for the fish swimming testing device; testing the flow velocity induction of the fish; counting the testing data. The method for testing the flow velocity induction of the fish has the advantages that the problem of testing the flow velocity induction of echeneis naucrates can be solved, and the velocity tested by the method is adopted in the fishway design, which helps echeneis naucrates flow through the fishway.