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发明授权
CN104480105B 藏菖蒲快速鉴定方法 失效 - 权利终止

藏菖蒲快速鉴定方法
摘要：
本发明公开了一种藏菖蒲快速鉴定方法，其包括如下步骤：1)以待测样品DNA为模板，扩增含有SEQ ID No.1所示的序列；2)对扩增产物进行测序，分析SEQ ID No.1所示的序列，如果自SEQ ID No.1所示序列的5’端起第23位为C和/或第212位为G，则鉴定为藏菖蒲，如果自SEQ ID No.1所示序列的5’端起第23位为T、第212位为C或T，则待鉴定样品不是藏菖蒲。本发明能够实现藏菖蒲原植物及其近缘种、药材、生饮片和混淆品的快速、准确鉴定。
摘要（英）：

The invention discloses a rapid identification method of Rhizoma Acori Calami. The method comprises the following steps: 1) DNA of a sample to be tested is used as a template, and fragments containing sequence as shown in SEQ ID No.1 are amplified; and 2) amplification products are sequenced, and the sequence as shown in SEQ ID No.1 is analyzed; if the 23rd site from 5' end of the sequence as shown in SEQ ID No.1 is C and/or the 212th site is G, the sample is identified as Rhizoma Acori Calami; and if the 23rd site from 5' end of the sequence as shown in SEQ ID No.1 is T and the 212th site is C or T, the sample to be identified is not Rhizoma Acori Calami. By the above method, rapid and accurate identification of the Rhizoma Acori Calami orginal plant and its sibling species, medicinal materials, raw medicinal slices and adulterants can be realized.

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