The invention belongs to the field of traditional Chinese medicines, discloses a composition as well as a preparation method and application thereof, and in particular discloses a composition for improving menopausal syndrome symptoms as well as a preparation method and application thereof. The composition disclosed by the invention is prepared from raw materials manyprickle acanthopanax root, a soybean extract, vitamin B2 and vitamin B6, the formula of the composition is simple, and the raw materials affect one another with synergistic reaction, so that the composition has an anti-oxidation effect, can supplement estrogen, and has a good effect of improving symptoms of dysphoria, insomnia during the age critique of women. A preparation method of the composition disclosed by the invention is simple to operate and is suitable for industrial production.