The invention provides a traditional medicine composition for livestock as well as a preparation method and application thereof. The traditional medicine composition disclosed by the invention is a preparation prepared by taking raw medicine powder or an extract of the root of Chinese pulsatilla and cortex ailanthic as active components and adding pharmaceutically acceptable auxiliary materials or auxiliary components. A pharmacodynamic test proves that by combining the root of chinese pulsatilla and the cortex ailanthic at a specific ratio in use, the traditional medicine composition disclosed by the invention has a synergistic interaction effect and has the advantage of enhancing the treatment effects of clearing away heat and toxic materials, promoting diuresis to eliminate stagnation, cooling blood and relieving dysentery. Besides, the invention also provides the preparation method of the medicine composition, wherein traditional Chinese medicine extracts are taken as raw materials so that the technique is more standardized, the process is easy to control, the operation space can be remarkably saved by adopting a preparation mode of a concentrate extract, convenience is provided for preservation of raw medicines, and an obvious technical advantage is achieved. In an optimal technical scheme, a small amount of azedarach and pomegranate bark are added for enhancing an antimicrobial effect to a certain extent so that the traditional medicine composition disclosed by the invention is remarkable in treatment effect when used for treating intestinal tract bacterial infection of livestock.