The invention relates to a cylinder clamp for machining of a carding machine cylinder. The cylinder clamp comprising a clamp body is characterized in that the front of the clamp body is provided with a clamping groove used for clamping a comb-tooth portion of the cylinder, one side of the clamping groove is provided with a vertical face, the upper edge of the other side of the clamping groove is provided with an overhanging protrusion horizontally extending towards the inside of the clamping groove, and a fastening groove is provided below the overhanging protrusion. The cylinder clamp is simple in structure and is specially used for clamping the carding machine cylinder, other combined clamps complex in structure are not required, and machining performance and precision of a four-axis linkage vertical machining center can be improved.