A carzenide high performance liquid chromatography measuring method belongs to high performance liquid chromatography measuring methods. The method uses an Agilent 1200 high performance liquid chromatography instrument. The chromatographic conditions includes that a chromatographic column is a C18 reverse phase column, a sodium dihydrogen phosphate buffer solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, an isocratic elution method is used, the flow rate of the mobile phase is 1 mL/min, the detection wavelength is 230 nm, the column temperature is 25 DEG C and the the injection volume is 20 muL/min. An oxidation product of toluenesulfonamide is filled into the liquid chromatography instrument, toluenesulfonamide and carzenide are subjected to simultaneous qualitative and quantitative detection according to the retention time and the peak area of a chromatographic peak, and further analysis is performed to obtain the yield and selectivity of carzenide obtained through toluenesulfonamide oxidation synthesis. The carzenide high performance liquid chromatography measuring method is high in accuracy and sensitivity, quick, easy and comprehensive in analysis and provides an effective detection tool for process optimization of carzenide.