The invention discloses a personnel access safety detection system, and belongs to the field of personnel access safety check. The system comprises a detection channel, a first safety gate, a second safety gate, an iris identification module, a human body inductor, a gate control unit and a background server; the first safety gate and the human body inductor are both arranged at the inlet end of the detection channel, and the second safety gate and the iris identification module are both arranged at the outlet end of the detection channel; the gate control unit controls opening and closing of the first safety gate and the second safety gate respectively through two paths of output signals; the iris identification module sends detected iris image information to the gate control unit, and the signal output terminal of the human body inductor is connected with the input terminal of the gate control unit; and the background server is wirelessly connected with the gate control unit. By employing the iris identification manner to identify the identity of a person attempting to access, the system has the advantages of rapid identification speed and high identification accuracy.