The invention provides a running-in device for fishing reels. The running-in device comprises a machine stand, control switches, press fitting mechanisms, on-load air cylinder mechanisms, jacking mechanisms and quick changing mechanisms. Each jacking mechanism comprises a jacking air cylinder, a rotating stepping motor and an automatic guiding mechanism. Each automatic guiding mechanism comprises a first connecting block, a second connecting block, a connecting rod, a guiding spring and a rotating head, wherein one side of the first connecting block is connected with a rotating shaft of the rotating stepping motor, the other side of the first connecting block is connected with the second connecting block through the connecting rod, the connecting rod is sleeved with the guiding spring, the two ends of the guiding spring are connected with the first connecting block and the second connecting block respectively, the rotating head is connected with the other side of the second connecting block, and the rotating head is concentric with the rotating shaft of the rotating stepping motor. The running-in device is simple in structure, high in loosening and clamping speed, convenient and safe to use and suitable for batch production.