The invention discloses a preparation method and a product of a powdery comprehensive enzyme strain, and discloses a preparation method and a product of a comprehensive enzyme bacterial fertilizer prepared with the powdery comprehensive enzyme strain. According to the invention, an active beneficial flora suitable to grow in slightly acidic red and brown clayed soils is added into an existing enzyme flora; with active enzyme collected and extracted from buffalo saliva, and through subculture and expanding propagation with nutrient solution, when the fertilizer is applied into red and brown clayed soils, organic matter decomposition can be effectively accelerated, beneficial bacteria unit number can be increased, carbon dioxide element conversion is correspondingly enhanced, and inorganic salt fertilizer dose is reduced. Therefore, soil can be improved and activated, fertilizer effect can be enhanced, plant root, stem and leaf growth can be promoted, crop quality can be greatly improved, and doses of chemical fertilizers and other fertilizers can be reduced. The preparation methods and the products are suitable for popularization.