The invention provides a three-phase tube bus vertical structure of a 1000 kV transformer substation. The three-phase tube bus vertical structure comprises three-phase tube buses, tube bus fixing fittings and supports, wherein the three-phase tube buses are connected with the independent tube bus fixing fittings receptively, the supports are connected with the tube bus fixing fittings and the tube buses in an insulated mode, the planes where the three-phase tube buses are located are all perpendicular to the horizontal ground, and the phase spacing between every two adjacent tube buses ranges from 1.7 m-2.5 m. On the premise that safe operation of the 1000 kV transformer substation is achieved, the overall width of the three-phase tube buses can be reduced to about 1.85 m from about 4 m of a conventional horizontal structure, and accordingly the overall length or width of the 1000 kV transformer substation can be reduced by about 2.15 m, so that the occupied area of the 1000 kV transformer substation is effectively reduced without affecting installation, operation and maintenance of equipment, precious land resources are saved, construction difficulty of the 1000 kV transformer substation is easily lowered, and operating maintenance of the 1000 kV transformer substation is more convenient.