The invention discloses a mutation breeding method for cunninghamia lanceolata. The method comprises the following steps: especially adopting ripen seeds of a cunninghamia lanceolata seed garden; carrying out low-temperature overwintering treatment, carrying out constant-temperature seed soaking stimulation on a gibberellin solution with specific concentration, immediately putting the gibberellin solution subjected to constant-temperature seed soaking stimulation into clear water, carrying out variable-temperature soaking for 3 days, and carrying out sprout promoting treatment; respectively carrying out colchicine mutation treatment, ethylmethane sulfonate (EMS) mutation treatment and sodium azide (NaN3) mutation treatment; and finally seeding and germinating the cunninghamia lanceolata seeds which are subjected to mutation treatment, so as to obtain a large group of cunninghamia lanceolata mutation seedlings. The cunninghamia lanceolata mutation seedlings obtained by the mutation method have the characteristics of obvious phenotypic characteristic variation, large population growth characteristic variation range and the like; a great number of mutated cunninghamia lanceolata materials can be formed within a short period of time by the method disclosed by the invention; and a foundation is laid for breeding of a new variety of cunninghamia lanceolata.