The invention discloses an application of a naphthalene-1,4-diketone compound used as a hCBS enzyme inhibitor and specifically relates to an application of a compound 2-chloro-3-(4-methyl piperidine-1-yl)naphthalene-1,4-diketone used as a hCBS enzyme inhibitor. IC50 of the small molecule compound for in vitro hCBS enzymatic reaction is 20 micro mole. The small molecule compound can be used as a tool drug for researches on H2S signal channels as well as a lead compound for development of a medicine for treating H2S-related diseases such as circulatory shock, apoplexy, Down's syndrome, tumor and the like.