The invention discloses a two-segment-charged alkylation reaction method. The alkylation reaction method comprises the steps: enabling concentrated sulfuric acid and isobutane to enter a colloid mill, and thoroughly mixing, so as to form an emulsion; enabling the emulsion and a hydrocarbon mixture containing C3-C5 alkene and isobutane to enter an alkylation reactor for reaction, wherein the reactor comprises a tube body, closure heads, an inner revolving bed, an outer revolving bed, a charging pipe, a charge distribution pipe, a discharging opening, a circulating cooling gas inlet and a circulating cooling gas outlet; the inner revolving bed is connected with an upper driving device through the charging pipe, the upper end of the inner revolving bed and the charge distribution pipe are fixedly connected into an integrated structure through a sealing member, the upper part of the outer revolving bed and a reactor shell body are fixedly connected through a sealing member, and the outer revolving bed is connected with a lower driving device through a revolving shaft. The alkylation reaction method disclosed by the invention has the advantages that the degree of mixing of isobutane and concentrated sulfuric acid can be greatly increased, the reaction can be carried out at relatively low temperature, the acid consumption of the reaction is low, the quality of products is high, and the equipment scale is small.