The invention provides a calculation method of profile control agent particle concentration, and belongs to the technical field of water injection well profile control water shutoff in oil field development process; the method takes consideration of parameters including water injection pressure difference, stratum porosity, stratum permeability, profile control agent expansion times, as well as scheduled profile control depth, and the like; the method comprises the following steps: 1) collecting data; 2) calculating stratum average pore diameter; 3) designing mass concentration of each stage of particles; 4) designing particle diameter mid-value of the first stage of particles; 5) designing particle diameter mid-value of the second stage of particles. The calculation result of the provided calculation method is relatively precise; the method can guide the actual profile control construction better; proper profile control agent particle concentrations and diameters can be designed for different sandstone stratums; meanwhile, the calculation formula can be easily programmed by computers.