The invention discloses a PCB design layout audit system, the PCB design layout audit system comprises an automatic DFM audit module and a man-machine interaction audit module; the automatic DFM audit module comprises a line audit module, a via hole audit module, a copper sheet audit module, a silk screen audit module, a solder resist audit module and a steel mesh audit module; the man-machine interaction audit module comprises a netlist audit module, a layout audit module, a signal audit module, a structure IDF output module, a device height reporting module, a locating and wiring related interaction audit module, a silk screen related interaction audit module and a production related interaction audit module. The audit system is the PCB audit tool based on the cadence allegro design platform, the system has ipc interaction communication of the allegro skill program via the windows program, the system integrates the automatic DFM audit function and the man-machine interaction audit function for having audit on the design result of PCB conveniently, the once success rate of the PCB design is raised and the integral development cycle and development cost of the electronic products are shortened.