The invention provides an information security protection system and method for a video monitoring system. The information security protection system comprises a server management module, a built-in protection module, a USB protection module, a network protection module and an operating seat protection module, performs security protection in four aspects, namely, a video monitoring center, a user terminal, a USB storage interface and networking of the video monitoring system, respectively, timely encrypts video files downloaded and exported from the video monitoring system as well as picture files produced by screenshot tools, performs security control on screenshots and screen records of operating systems during operations of the video monitoring center and the user terminal, and performs real-time monitoring on the network of the video monitoring system and the USB storage interface. The system and the method can provide data security protection for the video monitoring system and are complete in protection, high in extendibility, high in security, low in system resource occupation rate and good in user experience in terms of operation behavior.