The invention relates to a traditional Chinese medicine composition for prevention and control of tobacco insect pests. The composition comprises the mixture of ethanol extracts and supercritical extraction volatile oil of the following Chinese herbs in parts by weight: 1-2 parts of star anise, 2-3 parts of pepper, 1-2 parts of lemon, 2-3 parts of purple perilla and 1-2 parts of litsea auriculata. The invention also relates to a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition provided by the invention has a remarkable synergistic effect, and can be used for repelling and killing tobacco storage insect pests. Prevention and killing effects on insect pests are more than 80%, the traditional Chinese medicine composition is effective within 8 months, has no residual toxicity and is applicable to prevention and control of tobacco storage insect pests, plentiful raw materials are available, the production technology is simple, and the production cost is low.