The invention provides a bottle isolating air from the remaining liquid in the bottle after liquid pouring, which has the advantages that after part of liquid in the bottle is poured out, air entering the bottle cannot touch the rest of the liquid in the bottle, so that the rest of the liquid in the bottle is prevented from deteriorating due to being in contact with the air, and longer storage is convenient. The isolation bottle consists of a bottle body, an air inlet, an outflow port and an air bag; the air inlet and the outflow port are formed in a bottle opening; the air bag is communicated with the air inlet in the abdomen of the bottle body; the volume of the air bag after the air bag is filled up with air and spread is equal to the capacity of the bottle body; the air inlet is provided with an air inlet check valve for enabling air to inflow only and not to outflow; the outflow port is provided with an outflow port check valve for enabling the liquid in the bottle to flow out while air cannot enter the bottle body. When the liquid is poured from the outflow port, the air enters the air bag from the air inlet under the action of atmospheric pressure; when the bottle body is erected, the outflow port check valve prevents air from entering the bottle from the outflow port and the air inlet check valve prevents the air in the air bag from flowing out, and the air flowing into the bottle is contained in the air bag and is prevented from being in contact with the rest of the liquid in the bottle.