The present invention discloses a lipase enzyme activity detection fluorescence method. According to the present invention, the characteristic that the nature substance curcumin has fluorescence is utilized, the curcumin is adopted as the fluorescence signal element, and tween is concurrently adopted as the lipase substrate and the carrier for wrapping the signal molecule curcumin; the fluorescence analysis method with characteristics of low cost, simple operation, convenience and rapidness is developed; and the adopted tween is amphiphilic, the emulsification process is not required, and the signal molecule curcumin can be commercially obtained, such that the operation process is simplified, and the method is suitable for promotion and application.