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发明授权
CN104611466B 一种快速鉴别三种仔猪病毒性腹泻的分子试剂盒与应用 有权

一种快速鉴别三种仔猪病毒性腹泻的分子试剂盒与应用
摘要：
本发明公开了一种检测猪流行性腹泻病毒、猪传染性胃肠炎病毒和猪轮状病毒的试剂盒，它包括SEQ ID NO：1～2、SEQ ID NO：3～4和SEQ ID NO：5～6所示引物对，分别特异扩增猪流行性腹泻病毒、猪传染性胃肠炎病毒和猪轮状病毒的基因。本发明还公开了SEQ ID NO：1～2、SEQ ID NO：3～4和SEQ ID NO：5～6所示引物对在制备检测猪流行性腹泻病毒、猪传染性胃肠炎病毒和猪轮状病毒的试剂中的用途。本发明检测试剂盒可以准确、有效的检测猪流行性腹泻病毒、猪传染性胃肠炎病毒和猪轮状病毒，且特异性强、灵敏度高、耗时短，检测快速，应用前景良好。
摘要（英）：

The invention discloses a kit for detecting pig epidemic diarrhea viruses, pig transmissible gastroenteritis viruses and pig rotaviruses. The kit comprises primer pairs shown by SEQ ID NO:1-2, SEQ ID NO:3-4 and SEQ ID NO:5-6 for respectively performing specific amplification on the pig epidemic diarrhea viruses, the pig transmissible gastroenteritis viruses and the pig rotaviruses. The invention also discloses applications of the primer pairs shown by the SEQ ID NO:1-2, the SEQ ID NO:3-4 and the SEQ ID NO:5-6 in preparation of reagents for detecting the pig epidemic diarrhea viruses, the pig transmissible gastroenteritis viruses and the pig rotaviruses. The detection kit disclosed by the invention can be used for accurately and effectively detecting the pig epidemic diarrhea viruses, the pig transmissible gastroenteritis viruses and the pig rotaviruses, and is strong in specificity, high in sensitivity, short in time consumption, rapid in detection and good in application prospect.

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