The invention provides a three-dimensional dynamic monitoring method and a three-dimensional dynamic monitoring system of a machine room. The method comprises the following steps: building and showing a machine room three-dimensional model corresponding to the distribution of machine room equipment through a comprehensive manager; respectively building a connection relation among a machine room monitoring system, a video monitoring system and the comprehensive manager; receiving real-time monitoring data and real-time video monitoring data by the comprehensive manager and identifying on the equipment corresponding to the machine room three-dimensional model; judging whether the real-time monitoring data exceeds a preset threshold value; acquiring equipment information corresponding to the real-time monitoring data and camera information about the monitoring and the shooting of the equipment if the real-time monitoring data exceeds a preset threshold value; warning and prompting the corresponding equipment of the machine room three-dimensional model and displaying by automatically calling real-time video images of the camera information. According to the method and the system, three-dimensional integrated management of the machine room system is realized, and the machine room three-dimensional model is combined with video monitoring, so that business centralization is realized.