The invention discloses a water supply system for box-type culture. The water supply system comprises a combined pipeline arranged in a culture box and distributed in the length direction of the culture box. The combined pipeline comprises an outer pipe and an inner pipe which are both arranged at the top of the culture box. The combined pipeline is further provided with multiple nozzles communicated with the inner pipe and the outer pipe. The nozzles are arranged at equal intervals. One end of the outer pipe and one end of the inner pipe both extend out of the culture box. A sealing ring is arranged between the outer pipe and the culture box. One end of the outer pipe is connected with a nutrient solution box through a first hose. One end of the inner pipe is connected with a water tank through a second hose. The first hose is provided with a first delivery pump used for pumping a nutrient solution. The second hose is provided with a second delivery pump used for pumping water. By the adoption of the water supply system for box-type culture, through the combined pipeline composed of the inner pipe and the outer pipe, the space in the culture box is greatly saved, and the water supply system is simple in structure, convenient to use and convenient to use and popularize.