The invention provides a quick sterilization method of banana wilt soil-borne pathogenic bacteria (fusarium oxysporum f. sp. Cubense), and belongs to the technical fields of agricultural land governance and agricultural utilization. The method comprises the following steps: firstly adding an organic carbon source to continuous cropping soil that a continuous cropping obstacle occurs or the incidence of banana wilt is relatively high, and keeping the soil under an anaerobic state to create a strong reducing environment, reducing the oxidation-reduction potential of the soil to be less than minus 80mV within 72h and keeping the soil under the strong reducing environment for 2-4 weeks so as to obtain healthy soil which is relatively low in content of the fusarium oxysporum f. sp. Cubense. By creating the strong reducing environment, the quick sterilization method disclosed by the invention can quickly promote the growth and the metabolism of anaerobic microorganisms in the soil so as to generate a great amount of metabolites to effectively reduce the number of fusarium oxysporum in the affected soil; the method not only can improve the soil microflora but also can relieve the incidence of soil-borne diseases in crops; the method can increase the pH value of acid soil and improve the physiochemical properties of the soil; and the method is low in cost, easily available in materials, simple in operation and easy in popularization at grass-root level.