The invention discloses a chemical-luminescent enzyme-linked immunoassay kit for detecting bentazone. The kit includes a kit body, and an ELISA plate and a reagent which are arranged therein. The kit is characterized in that holes on the ELISA plate are coated by a conjugate of a coating antigen, namely, bentazone-type mother nucleus, and a carrier protein. The reagent includes: a bentazone-type monoclonal antibody, a horse radish peroxidase-marked goat-anti-mouse antibody, a bentazone-type series standard solution, a concentrated phosphate buffer liquid, a concentrated washing liquid and a chemical-luminescent liquid. The chemical-luminescent enzyme-linked immunoassay kit is high in sensitivity, is simple, convenient and quick, is high in accuracy and can detect various types of medicines. Compared with a conventional colorimetric ELISA method, a method of the kit is greatly reduced in operation time. The method can be used for directly detecting the content of the bentazone in crops (such as soya beans, rice, wheat, barley and the like).