The invention discloses a separator, a lithium battery including the separator, a method of manufacturing the separator, and a method of manufacturing the lithium battery. The separator includes an organic-inorganic hybrid coating layer on at least one surface of a porous base and a pattern coating layer on a surface of the organic-inorganic hybrid coating layer. The pattern coating layer includes patterns having an average diameter of 0.1 mm or less that are regularly spaced apart from each other.