The invention belongs to the technical field of assembly machinery, and particularly relates to a nail polish filling production line which comprises a frame, an empty bottle feeding device, a finished product collection line, an inner cover feeding device, an outer cover feeding device, an inner cover conveying line and an outer cover conveying line. A turntable is arranged in the frame and circumferentially provided with a clamping position for placing empty bottles, a conveying device is arranged between the empty bottle feeding device and the turntable, a roller assembly device, a nail polish filling device, an inner cover assembly device, an inner cover feeder, an outer cover screw-in device and an outer cover feeder are sequentially arranged along the circumferential direction of the turntable, and the roller assembly device is close to the conveying device. By the aid of the nail polish filling production line, nail polish is full-automatically filled, workers do not need to participate, a lot of labor is saved, and production efficiency is greatly improved.