The invention relates to an air acquisition box device. A battery, power supply wiring, elastic connection, a magnetically-controlled switch and a time regulator are fixedly mounted on an acquisition box fixator; and a fixing clip is mounted on an acquisition box fixator cover. When air needs to be acquired for culture, desired time is set by using the time regulator, and the acquisition box fixator automatically opens and automatically closes on time; when a culture dish needs to be taken away, the fixing clip on the acquisition box fixator cover is fixed to allow a culture dish cover and the culture dish to be fixed, so the culture dish can be taken away without being polluted; and under the condition of no alternating current source, the acquisition box fixator is powered up by the battery, so automatic acquisition of air specimens under any condition is realized and accuracy of air results is ensured.