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发明公开
CN104698167A 一种阪崎肠杆菌检测试剂及其制备方法 无效 - 驳回

一种阪崎肠杆菌检测试剂及其制备方法
摘要：
一种阪崎肠杆菌检测试剂及其制备方法，涉及一种细菌检测试剂及其制备方法。本发明是为了解决现有的检测阪崎肠杆菌的胶体金试纸条无法定量检测的问题。该检测试剂由试剂A和试剂B组成，试剂A包括缓冲液、促凝剂、BSA和防腐剂；试剂B包括标记有抗阪崎肠杆菌多克隆抗体的胶体金颗粒、稳定剂、缓冲液和防腐剂。方法：一、将促凝剂、BSA和防腐剂加入到缓冲液中，制得试剂A；二、将稳定剂、防腐剂和缓冲液混合得混合液，然后将混合液与标记有抗阪崎肠杆菌多克隆抗体的胶体金颗粒混合，制得试剂B；三、将试剂A和试剂B混合即得抗阪崎肠杆菌检测试剂。本发明检测线性范围大，可定量检测，分析灵敏度高，使用方便。用于检测牛奶或奶粉中阪崎肠杆菌。
摘要（英）：

The invention discloses enterobacter sakazakii detection reagent and a preparation method thereof and relates to bacterial detection reagent and a preparation method thereof. The enterobacter sakazakii detection reagent and the preparation method thereof are aimed to solve the problem that an existing colloidal gold test strip for detecting the enterobacter sakazakii cannot perform quantitative detection. The detection reagent is composed of reagent A and reagent B, and the reagent A comprises buffer solution, coagulation accelerator, BSA and preservative; the reagent B comprises colloidal gold particles labeled with anti-enterobacter sakazakii polyclonal antibody, stabilizing agent, buffer solution and preservative. The preparation method includes that firstly, adding the coagulation accelerator, BSA and preservative to the buffer solution to obtain the reagent A; secondly, mixing the stabilizing agent, preservative and buffer solution to obtain the mixed solution, and mixing the mixed solution with the colloidal gold particles labeled with anti-enterobacter sakazakii polyclonal antibody to obtain the reagent B; thirdly, mixing the reagent A with the reagent B to obtain the enterobacter sakazakii detection reagent. The enterobacter sakazakii detection reagent is large in detection linearity range, capable of performing quantitative detection, high in analysis sensitivity and convenient to use. The enterobacter sakazakii detection reagent is used for detecting the enterobacter sakazakii in milk or milk powder.

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