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发明公开
CN104711338A miR-24作为血浆/血清miRNA检测的内参基因的应用 无效 - 驳回

miR-24作为血浆/血清miRNA检测的内参基因的应用
摘要：
本发明公开了miR-24作为血浆/血清miRNA检测的内参基因的应用。本发明还保护一种检测血浆或血清中miRNA的相对表达量的方法，包括如下步骤：（1）提取待测血浆或血清的总RNA并反转录为cDNA；（2）以步骤（1）获得的cDNA为模板，分别检测其中目的miRNA的编码序列的含量和miR-24的编码序列的含量；（3）根据步骤（2）的结果，以所述miR-24为内参基因，计算目的miRNA的相对表达量。采用miR-24为内参基因可以实现不同实验室间研究结果的比较，促进各实验结果的归一化处理，进而促进血清/血浆miRNA研究结果向临床成果转化，为临床医生提供更加精确的诊断、分型、预后判断以及个体化治疗的有力工具。
摘要（英）：

The invention discloses a use of miR-24 as a reference gene of blood plasma/serum miRNA detection. The invention also protects a method for detecting a relative expression amount of miRNA in blood plasma/serum. The method comprises the following steps of 1, extracting total RNAs in blood plasma/serum to be detected and carrying out inverse transcription to obtain cDNAs, 2, respectively detecting target miRNA coding sequence content and miR-24 coding sequence content by the cDNAs as templates obtained by the step 1, and 3, according to the result obtained by the step 2, calculating a target miRNA relative expression level by the miR-24 as a reference gene. The miR-24 is used as a reference gene so that comparison between research results obtained by different laboratories is realized, and an experiment result normalization process is promoted and thus the use of the miR-24 promotes conversion from the blood plasma/serum miRNA research results into clinical results and provides a powerful tool used for accurate diagnosis, parting, prognosis judgment and individual treatment for clinical doctors.

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