The invention discloses a safety protection method of an OPC protocol based on a dynamic tracking technology. One end of the method is connected with one or more OPC client-sides, the other end of the method is connected with one or more OPC servers, through designed data capturing and an OPC package of analytical analysis of a data package, then dynamic tracking and authorization management of an interface are conducted, the content of the interface of the OPC data package is tracked, after connection is established, the flowing data packages are subjected to monitoring based on the interface and the protocol, and illegal data packages are prevented from passing. According to the method, equipment is just needed to be accessed between the OPC client-sides and OPC server-sides, other configuration operation is not needed, and the security of an OPC communication network is guaranteed.