The invention provides a method for purification of citric acid from citric acid fermentation liquid, the method comprises the following step of use of an anion exchange resin chromatography system for chromatographic separation of the citric acid fermentation liquid for removal of residual sugar to obtain purified citric acid, and the anion exchange resin particle size is 0.15-0.35mm. By controlling the anion exchange resin particle size to range between 0.15-0.35 mm, the elution agent dosage in the process of use of the anion exchange resin chromatography system for purification of the citric acid can be effectively reduced, the dilution degree of the isolated citric acid purification liquid can be effectively controlled, the separation efficiency can be improved, and the subsequent concentrate cost can be substantially reduced.