A process for using specific desulphurization mixed bacterium for high sulfur coal mine biological desulfurization is as follows: ore is crushed to-25mm for heap construction, the heap height is 8-10m, temperature and gas composition induction devices are embedded during heap construction; then spray operation is performed, the spraying intensity is 10-15L / (M<2>.H), the spray solution contains 107-108cfu / mL of specific desulphurization mixed bacterium CGMCC NO.8336, the oxidation reduction potential of the spray solution is more than 700mV (SCE), the spray operation is performed according to the mode of spraying for 7 days and resting for 5 days or spraying for 7 days and resting for 7 days, the in-heap temperature is controlled at 45 to 60 DEG C, and the leaching system pH value is 1.5 to 1.8. The process has the advantages of short process, simple equipment, small investment, low cost, no pollution, larger or smaller production scale, and capability of processing high sulfur coal mine resources which cannot be processed by traditional mineral processing and metallurgical technology, and can expand the resource utilization scope and improve the comprehensive recovery level of the coal mine resources.