利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104745637A 一种以微生物细胞分泌液为基质制备硒纳米颗粒的方法 无效 - 驳回

一种以微生物细胞分泌液为基质制备硒纳米颗粒的方法
摘要：
本发明公开了一种以微生物细胞分泌液为基质制备硒纳米颗粒的方法，属于纳米颗粒合成技术领域。本发明的技术方案要点为，一种以微生物细胞分泌液为基质制备硒纳米颗粒的方法，接种微生物单菌落至30-100mL水中，加入质量浓度为0.2%-1.6%的糖类溶液；震荡24h后加入1-5mmol的硒粉、硒酸或亚硒酸钠，然后将混合溶液放入恒温恒速摇床中，于20-60℃震荡3-24h，震荡速率为100-600r/min；离心收集沉淀，洗涤，干燥得到粒径3-8nm的硒纳米颗粒。本发明具有环境友好，生物相溶性好和制备过程简单等优点。
摘要（英）：

The invention discloses a method for preparing selenium nano-particles by taking secretion of microbial cells as a matrix, belonging to the technical field of synthesis of nano-particles. The method for preparing selenium nano-particles by taking secretion of microbial cells as a matrix is technically characterized by comprising the following steps: inoculating 30-100mL of water with microbial single colonies and adding a sugary solution with mass concentration of 0.2-1.6%; oscillating for 24h, then adding 1-5mmol of selenium powder, selenic acid or sodium selenite, putting the mixed solution in a constant-temperature constant-speed shaker and oscillating for 3-24h at 20-60 DEG C at an oscillating speed of 100-600r/min; and centrifuging, collecting precipitate, washing and drying to obtain selenium nano-particles with particle size of 3-8nm. The method disclosed by the invention has the advantages of environment friendliness, good biocompatibility, simple preparation process and the like.

0/0